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货号	名称	规格	价格(元)	说明书
A3901-01	Bst 3.2 DNA Polymerase(冻干品)	800U	300
		4KU	1,250
D0303-02	Max65反转录酶(冻干品)	5KU	300
		25KU	1,200

         Bst3.2 DNA聚合酶经酶电子重构架和进化筛选（in silico Design & invitro Evolution），将Bst2.0和Bst3.0特性融合，保留了3.0的快速扩增能力和增强的内置RT活性，融合了2.0的扩增特异性佳和dUTP使用能力（允许使用50%的dUTP来代替dTTP）。该酶具有5’-3’的DNA聚合酶活性和强烈的链置换活性，但其5’-3’和3’-5’外切酶活性缺失。
         Bst3.2 DNA聚合酶用于RT-LAMP实验，尽管Bst3.2 DNA聚合酶本身具有良好内置的RT活性，但在低Copy样本的RT-LAMP中表现出反应速度滞后，因此在RT-LAMP反应时建议搭配耐热逆转录酶Max65 RTase（HaiGene D0303-02），该组合方案下Bst3.2 DNA聚合酶的使用性能等同于HaiGene的Bst4.0 DNA/RNA聚合酶，该酶是Bst4.0 DNA/RNA聚合酶的核心组分。

性能展示

Bst3.2 DNA聚合酶对RNA模板的扩增能力展示:分别用Bst 2.0(0.32U/μl)、3.2(0.32U/μl)、3.2(0.32U/μl)+Max65 RTase(2U/μl)，以A549细胞Total RNA为模板，进行RT-LAMP扩增。

应用产品文献

1. Rapid Visual Detection of Elite Erect Panicle Dense and Erect Panicle 1 Allele for Marker-Assisted Improvement in Rice (Oryza sativa L.) Using the Loop-Mediated Isothermal Amplification Method. Curr Issues Mol Biol. 2024 Jan 4;46(1):498–512.
2. Rapid Visual Detection of High Nitrogen-Use Efficiency Gene OsGRF4 in Rice (Oryza sativa L.) Using Loop-Mediated Isothermal Amplification Method. Genes (Basel). 2023 Sep 23;14(10):1850.
3. Rapid and visual identification of HIV-1 using reverse transcription loop-mediated isothermal amplification integrated with a gold nanoparticle-based lateral flow assay platform. Front Microbiol. 2023 Jul 12;14:1230533.
4. Rapid Detection of Streptococcus mutans Using an Integrated Microfluidic System with Loop-Mediated Isothermal Amplification. J Microbiol Biotechnol. 2023 May 19;33(8):1101–1110.
5. Rapid point-of-care detection of BK virus in urine by an HFman probe-based loop-mediated isothermal amplification assay and a finger-driven microfluidic chip.PeerJ. 2023 Mar 8;11:e14943.
6. Rapid detection of monkeypox virus and monkey B virus by a multiplex loop-mediated isothermal amplification assay. J Infect. 2023 Feb 13;86(4):e114–e116.
7. SMART: A Swing-Assisted Multiplexed Analyzer for Point-of-Care Respiratory Tract Infection Testing. Biosensors (Basel). 2023 Feb 4;13(2):228.
8. An HFman Probe-Based Multiplex Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Simultaneous Detection of Hantaan and Seoul Viruses. Diagnostics (Basel). 2022 Aug 10;12(8):1925.
9. Real-Time Detection of LAMP Products of African Swine Fever Virus Using Fluorescence and Surface Plasmon Resonance Method. Biosensors (Basel). 2022 Apr 3;12(4):213.
10.Multiplex, Real-Time, Point-of-care RT-LAMP for SARS-CoV-2 Detection Using the HFman Probe.ACS Sens. 2022 Feb 22;7(3):730–739.
11.Deep‐dLAMP: Deep Learning‐Enabled Polydisperse Emulsion‐Based Digital Loop‐Mediated Isothermal Amplification. Adv Sci (Weinh). 2022 Jan 24;9(9):2105450.
12.Development of a TaqMan loop-mediated isothermal amplification assay for the rapid detection of pigeon paramyxovirus type 1. Arch Virol. 2021 Mar 23;166(6):1599–1605.
13.Rapid, visual, label-based biosensor platform for identification of hepatitis C virus in clinical applications. BMC Microbiol. 2024 Feb 28;24:68.
14.A novel extraction-free dual HiFi-LAMP assay for detection of methicillin-sensitive and methicillin-resistant Staphylococcus aureus.Microbiol Spectr. 2024 Feb 20;12(4):e04133-23.
15.A duplex HiFi-LAMP assay for screening of two novel human circoviruses HCirV-1 and HCirV-2. BMC Infect Dis. 2024 Dec 5;24:1388.
16.Point-of-care test of blood Plasmodium RNA within a Pasteur pipette using a novel isothermal amplification without nucleic acid purification. Infect Dis Poverty. 2024 Oct 31;13:80.
17.Rapid detection of human influenza A viruses by HFman probe-based loop-mediated isothermal amplification assays. Heliyon. 2023 Oct 25;9(11):e21591.