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货号
名称
规格
价格(元)
说明书
TAPXXXXX HG TaqMan miRNA定量PCR试剂盒100次×20μl600
TAP01501 HG TaqMan RNU6B定量PCR试剂盒100次×20μl600
TAP01511HG TaqMan miR-16定量PCR试剂盒100次×20μl600
请于此文件中查询对应试剂盒货号TaqMan miRNA qPCR kit.xls
          探针法做为microRNA定量研究的金标准,其具有高特异性、高灵敏度的优势。HaiGene的HG TaqMan miRNA定量PCR试剂盒,采用特异性的正向miRNA引物和接头引物进行PCR扩增,检测荧光基团采用FAM标记的TaqMan探针。使用该试剂盒可以高特异性、高灵敏度的检测低至100个细胞的miRNA分子,并可区分相似度极高的miRNAs。HaiGene的TaqMan miRNA定量PCR试剂盒多达一万余种,试剂盒编号为TAPxxxxx,每一个mircoRNA分子对应一个检测试剂盒(包含特异性的TaqMan探针、正向引物、反向引物、定量试剂),可于HG TaqMan miRNA qPCR kit.xls中查询。
         特别说明:如您研究的miRNA不在我们的TaqMan miRNA qPCR Kit列表之中,请登录www.mirbase.org进行miRNA查询,并来信告知Accession No.和序列,我们将在2周内完成试剂盒的定制。
taqman mirna反转录试剂盒
组分
  组分名称 25T/100T
5×Golden HS TaqMan qPCR Mix 400 μl
20×miRNA TaqMan Assay 100 μl
储存
-20℃可保存2年,避免反复冻融。
功能测试
(该实验结果来自HaiGene实验室,未经允许,不得转载)

1. 高灵敏度 (最高可到0.1fM级水平)

mirna定量PCR试剂盒

2.高特异性,有效区分单碱基差异

mirna定量PCR试剂盒

3.应用实例

mirna定量PCR试剂盒
参考文献
[1] Development of a robust, low cost stem-loop real-time quantification PCR technique for miRNA expression analysis. Mol Biol Rep. 2013. PMID: 23307300.
[2] Quantitative stem-loop RT-PCR for detection of microRNAs. Methods Mol Biol. 2011. PMID: 21533691.
[3] MicroRNA detection in prostate tumors by quantitative real-time PCR (qPCR). J Vis Exp. 2012. PMID: 22643910.
[4] Circulating microRNA-196a as a candidate diagnostic biomarker for chronic hepatitis C. Mol Med Rep. 2015. PMID: 25738504.
[5] Novel real-time PCR assay of microRNAs using S-Poly(T), a specific oligo(dT) reverse transcription primer with excellent sensitivity and specificity. PLoS One. 2012. PMID: 23152780.
[6] Diagnostic microRNA markers to screen for sporadic human colon cancer in stool: I. Proof of principle. Cancer Genomics Proteomics. 2013. PMID: 23741026.
[7] Stem-loop RT-qPCR for microRNA expression profiling. Methods Mol Biol. 2012. PMID: 22144190. [8] Serum microRNA-21 as marker for necroinflammation in hepatitis C patients with and without hepatocellular carcinoma. PLoS One. 2011. PMID: 22066022.