Taq FS DNA Polymerase (5 U/μl) 0.25 μl
110×Taq FS PCR Buffer 10 μl
dNTP Mixture (2.5 mM each) 2 μl
*1模板DNA *X μl
引物F(10 μM) 2 μl
引物R(10 μM) 2 μl
ddH2O upto 50 μl
| 循环数 | 温度 | 时间 |
| 1st Cycle | 95°C | 2min |
| 25-35 Cycles | 95°C | 20s |
| Tm | 20s | |
| 72°C | 1kb/1min | |
| Last Cycle | 72°C | 2min |